U.S. population data for human identification markers

This information and data are supplemental files associated with: Gettings, K.B., Borsuk, L.A., Steffen, C.R., Kiesler, K.M., and P.M.Vallone. Sequence-based U.S. population data for 27 autosomal STR loci, Forensic Science International Genetics (submitted for publication). The primary data consists of sequence-based allele frequencies for N=1036 anonymized U.S. population samples at 27 autosomal Short Tandem Repeat (auSTR) loci: D1S1656, TPOX, D2S441, D2S1338, D3S1358, D4S2408, FGA, D5S818, CSF1PO, D6S1043, D7S820, D8S1179, D9S1122, D10S1248, TH01, vWA, D12S391, D13S317, Penta E, D16S539, D17S1301, D18S51, D19S433, D20S482, D21S11, Penta D, and D22S1045. This information is expected to support the implementation of sequence-based STR analysis in forensic applications. "NIST1036_auSTR_Seq_SuppTables.xls" is an excel file containing the following worksheets: run metrics for the 42 sequencing runs performed to generate the allele frequency data (Supp Table 1 - Run Metrics); coverage per locus per sample for all N=1036 at the 27auSTR, 7XSTR, and 24 YSTR loci reported by the manufacturer in this assay (Supp Table 2 - Coverage); allele frequency data (Supp Table 3 - Frequencies); GRCh38 reference coordinates for genomic regions reported in the 27 auSTRs (Supp Table 4 - Ref Coordinates); summary of polymorphisms detected and reported in STR flanking regions (Supp Table 5 - Flank Polymorph); number of alleles, expected and observed heterozygosity, and p-values associated with HWE testing by population for the 27 auSTR loci (Supp Table 6 - Hexp_Hobs_pHW); p-values associated with testing for linkage disequilibrium (Supp Table 7 - LD p-values); and pairwise Fst values by population for the 27 auSTR loci (Supp Table 8 - Pairwise Fst). Lastly, "NIST1036_auSTR_Seq_SuppFile1.pdf" contains information on optimization, sequencing, and quality control of the data.