rCRUX Generated trnl plants Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: trnl plants<br> Gene: trnl<br> Length of Target: ~85<br> get_seeds_local() minimum length: 60<br> get_seeds_local() maximum length: 110<br> blast_seeds() minimum length: 23<br> blast_seeds() maximum length: 73<br> max_to_blast: 250<br> Forward Sequence (5'-3'): GGGCAATCCTGAGCCAA<br> Reverse Sequence (5'-3'): TTTGAGTCTCTGCACCTATC<br> Reference: Coissac, E., Pompanon, F., Gielly, L., Miquel, C., Valentini, A., Vermat, T., ... &amp; Willerslev, E. (2007). Power and limitations of the chloroplast trnL (UAA) intron for plant DNA barcoding. Nucleic Acids Research 3 (35),.(2007). https://doi.org/10.1093%2Fnar%2Fgkl938 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.