MTT raw data for respective ganoderma chromatography fractions

Ganoderma is a genus of medically important fungus that contains at least
80 species, many of which have not been properly evaluated for their
anticancer potential.  This study was conducted to assess the
cytotoxic activity of the mycelial biomass of Ganoderma
weberianum-sichuanese isolated from the Lower Volta River Basin of
Ghana.  The nuclear ribosomal internal transcribed spacer (ITS)
region was analyzed to determine the phylogenetic position of this native
ganoderma isolate. We then tested its cytotoxic activity against the human
carcinoma cell line PC-3 (human prostate), Jurkat (human T lymphoblastoid
cell line), derived from an acute T cell leukemia, and PMDC05, a
plasmacytoid dendritic cell (pDC) derived from acute leukemia using the
3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT)
assay. The ITS phylogenetic analysis demonstrated that this native
Ghanaian ganoderma isolate belongs to the Ganoderma weberianum-sichuanese
species complex.  Fractions of the mycelial biomass were found to
inhibit significantly (≤ 0.05%) the proliferation and survival of the
three cancer cell lines, PC-3, PMDC05, and Jurkat with increasing
concentrations and with IC50 values of 27.73 ± 5.25, 21.31 ± 2.40 and
17.09 ± 0.86 μg/mL, respectively compared to Chang liver cells (CVCL_0238)
with an IC50 value of 75.41 ± 1.95 μg/mL. To the best of our knowledge,
these findings demonstrated for the first time, that specific constituents
of Ganoderma weberianum-sichuanese are selectively cytotoxic to the three
human cancer cell lines, suggesting their potential efficacy in the
treatment of malignancies. Future studies to isolate and characterize the
biologically active molecules are warranted.