Revealing the clinical impact of MTOR and ARID2 gene mutations on MALT lymphoma of the alimentary canal using targeted sequencing

To better understand the genetics of this tumor, targeted sequencing in a group of 31 cases (including 2 esophageal, 2 colonic, 4 small intestinal, and 23 gastric cases) were performed. Thirty-one specimens of MALT lymphomas and two lymph node hyperplasia specimens in different parts of the gastrointestinal tract diagnosed and treated at the Affiliated Hospital of Zunyi Medical University from January 2017 to October 2021 were collected, and the proportion of tumor cells estimated using morphology and immunohistochemistry was greater than 75% in all tumor cases. Target libraries were designed by using the library building kit named VAHTS AmpSeqLibrary Prep Kit V3NA210 and the lymphoma-associated 101 gene primer panel, and after checking the library quality, the sequencing template preparation was completed on an Ion Chef instrument (Thermo Fisher Scientific) and sequencing was carried out on an Ion GeneStudio S5 Plus instrument (Thermo Fisher Scientific) using a 200 bp read length. The panel contains the entire exome of 101 genes associated with lymphoma.