Free-Flow Isoelectric Focusing for Comprehensive Separation and Analysis of Human Salivary Microbiome for Lung Cancer

Human microbiome contains billions of microorganisms thatplay important roles in the biological system and different diseases. Due to itscomplexity, conventional culture-independent technology may underestimatethe value of low-abundance bacteria, which calls for a highly efficient methodfor its enrichment and comprehensive analysis. In this study, we developed arecycling free-flow isoelectric focusing (RFFIEF) method-based electrophoresismethod to separate human salivary microbiome.