BCCM/ULC Cyanobacteria Collection

BCCM/ULC is a small and dedicated public collection, currently containing one of the largest collections of documented (sub)polar cyanobacteria worldwide. The BCCM/ULC collection is hosted by the Centre for Protein Engineering (the Unit) of the University of Liège. The host Unit is very active in research projects concerning the cyanobacterial diversity and biogeography, with a focus on polar biotopes. The approach used is polyphasic, including the isolation of strains and culture-independent methods (DGGE, clone libraries, pyrosequencing based on the ribosomal operon sequences). The participation to field expeditions in the Antarctic and Arctic has enabled to collect samples in many locations. Moreover, taxonomic research is carried out by the host Unit to improve the classification of the cyanobacterial phylum. It is based on a polyphasic approach combining the morphological and molecular characterizations of the strains.

BCCM/ULC是一家小型专精的公共蓝细菌保藏库，目前馆藏了全球范围内规模最大的经鉴定（亚）极地蓝细菌菌株集合之一。该保藏中心由列日大学蛋白质工程研究单元托管。此研究单元在蓝细菌多样性与生物地理学相关研究项目中十分活跃，研究聚焦于极地生境。其采用的研究方法为多相分类法，涵盖菌株分离技术与非培养依赖型分析手段，包括变性梯度凝胶电泳（DGGE）、克隆文库构建以及基于核糖体操纵子序列的焦磷酸测序。参与南极与北极的野外考察工作，使得该团队得以在诸多采样点位采集到环境样本。此外，托管单元还开展分类学研究以完善蓝细菌门的分类体系，该研究依托结合菌株形态学表征与分子特征鉴定的多相分类方法。

搜集汇总

背景与挑战

背景概述

BCCM/ULC蓝藻收藏库是一个公共收藏库，目前拥有全球最大的已记录（亚）极地蓝藻菌株收藏之一，专注于极地蓝藻的多样性、生物地理学和生态生理学研究。该收藏采用多相方法（包括形态学和分子表征）对菌株进行系统分析，并已通过ISO 9001:2015质量认证，提供超过500株菌株作为活培养物或纯化基因组DNA。