Transcriptomic profiles of resected 50 pancreatic adenocarcinoma samples

An aggregated retrospective database with standardized clinicopathological variables was created for patients resected in Erasme and Pitié Salpêtrière hospitals. RNA was extracted from the scrapped sections with the ALLPrep FFPE tissue kit© following the manufacturer’s instructions for semi-automated RNA extraction via Qiacube instrument (Qiagen, Venlo, The Netherlands). RNA samples were run on an Agilent 2100 bioanalyzer using the RNA 6000 Pico LabChip kit (Agilent, Diegem, Belgium). The bioanalyzer electropherograms were analyzed by Agilent 2100 Expert Software to determine the RNA quantity and quality. RNA samples with DV200 >30% were selected and 100 ng of RNA was used for the library preparation. NGS libraries were prepared using the QuantSeq Library Prep Kit for Illumina (Lexogen) as per manufacturer recommendations’. The libraries were sequenced on NovaSeq using NovaSeq 6000 S2 Reagent Kit with 100 bp single reads.