S. neglecta parasitism in O. nigricornis: 2018 collection

In July 2018, we collected O. nigricornis crickets in southern Ontario (Canada) and New York State (United States of America): from the Koffler Scientific Reserve (coded as K or site 2) (King City Ontario, 44.03°N, 79.53°W), rare Charitable Research Reserve (coded as R or site 4) (Cambridge Ontario, 43.38°N, 80.39°W), the University of Toronto at Mississauga (coded as M or site 3) (43.55°N, 79.66°W), and the Edmund Niles Huyck Preserve and Biological Research Station (coded as H or site 1) (Rensselaerville, New York, 42.10°N, 74.10°W). 

Crickets were dissected and photographed using an AmScope 5MP digital microscope camera attached to a Wild M5A dissecting microscope. Following dissection, abdomens were examined for the presence of S. neglecta eggs and/or larvae. Crickets containing fly eggs and/or larvae were given a fitness score of 0, while those that did not contain either were given a fitness score of 1.

Crickets were photographed under the microscope and digital photos measured using ImageJ software. Measurements of pronotum length, femur length and head width were taken.