Gene expression of CD14+ peripheral blood monocytes in different phases of chronic HBV infection

To identify gene biomarkers for different phases of chronic HBV infection, we performed RNA-seq of CD14+ monocytes from pateints in each of the phases, which was then analyzed for differntial gene expression and validated by qRT-PCR in individual samples CD14+ monocytes were isolated by MACS from blood of HBV patients in Immune-tolerant (IT), Immune-clearance (IC) or inactive carrier (IN) and Healthy control (HC) and RNA was isolated from these cells. 1 sample from each of IT, IC, IN and HC were sequenced as single sample, while 5 samples of IT, IC and HC were pooled repectively, hence 7 sequenced samples. Differential gene expression compared to HC was generated after RNA-seq data analysis, hence 5 total lists of differntially expressed genes, which was shortlisted for PCR validation and analyzed.