Transcriptomic analysis of ethanol-pretreated Arabidopsis WT shoots under drought conditions

We report the differentially expressed genes between water-pretreated and ethanol-pretreated plants under drought conditions. Overall design: Arabidopsis thaliana (Col-0 ecotype) were grown on MS medium at 22°C under 16-h-light/8-h-dark for 9 days. Seedlings were then transferred to ceramics granular soil (SAKATA SEED CORPORATION, Japan) and grown for 5 days. Ethanol pretreatment was performed by immersing the pots in trays containing 10 mM Ethanol for 3 days. Drought treatment was then started by removing excess water from the trays and stopping subsequent watering. Shoots were harvested at 0, 3, 10 and 13 days after the onset of drought treatment.