Additional file 1 of Molecular repertoire of Deinococcus radiodurans after 1 year of exposure outside the International Space Station within the Tanpopo mission

Additional file 1: Figure S1. SEM images of LEO exposed and control cells. Scanning electron microscopy (SEM) images showing upper surface of multilayers of dehydrated cells of D. radiodurans deposited on aluminum plates. (a, c) SEM images of D. radiodurans cells exposed to LEO in Tanpopo mission. (b, d) SEM images of ground control cells of D. radiodurans. Figure S2. High magnification SEM images of LEO exposed cells. Higher magnification SEM images displaying upper surface of multilayers of dehydrated D. radiodurans cells after LEO exposure. Figure S3. SEM and TEM images after recovery. Scanning and transmission electron microscopy (SEM and TEM) images of D. radiodurans cells recovered after LEO exposure in complex medium. (a) SEM image of recovered D. radiodurans cells after LEO exposure. (b) TEM image of recovered D. radiodurans cells after LEO exposure. (c) SEM image of ground control D. radiodurans cells. (d) TEM image of ground control D. radiodurans cells. Figure S4. Statistical comparison of proteomics data between LEO exposed and control cells. (a) Protein hits present in all replicates, with a p-value below 0.05 identified in the extracellular compartment of LEO exposed and ground control cells. (b) Protein hits present in all replicates, with a p-value below 0.05 identified in the intracellular compartment. (c) PCA of all measured intracellular proteins. (d) Negative decadic logarithm of corrected p-values (q-values, y-axis) versus log2 fold change (x-axis) of all measured mRNAs. Transcripts with a q-value below 0.05 and a fold change >|1.5| are emphasized. (e) Abundance of proteases identified in the intracellular compartment. Significant differences are indicated with an asterisk (*). Figure S5. Gene Ontology annotation of higher abundant transcripts. Includes Gene Ontology annotation of molecular functions, biological processes, cellular components and protein classes of higher abundant transcripts with a q-value<0.05. Figure S6. Comparison of targeted metabolomics approaches. Both charts show the same metabolites. The upper one shows results after simulated LEO exposure of D. radiodurans (Sim_exp) compared with corresponding control (Sim_ctrl). The lower one shows results after the real LEO exposure experiment, comparing LEO exposed and ground control (Ctrl) cells. Table S1. LEO experiment - Table of raw extracellular protein LFQ intensities, corresponding statistical analysis, the number of identified unique peptides and the calculated Maxquant score. Table S2. LEO experiment - Table of raw intracellular protein LFQ intensities, corresponding statistical analysis, the number of identified unique peptides and the calculated Maxquant score. Table S3. LEO experiment - Table of calculated FPKM values and corresponding statistical analysis. Table S4. LEO experiment - Normalized values for targeted metabolites and corresponding statistical analysis. Table S5. LEO experiment - Normalized values for untargeted metabolites, corresponding statistical analysis and library search. Table S6. Simulation experiment - Normalized values for targeted metabolites and corresponding statistical analysis. Table S7. Simulation experiment - Table of raw intracellular protein LFQ intensities, corresponding statistical analysis, the number of identified unique peptides and the calculated Maxquant score.