<i>Rhizophora mucronata</i> Lam., a halophyte from Mauritius Island, inhibits the entry of Zika virus in human cells (A549)- an <i>in vitro</i> and <i>in silico</i> analysis
收藏资源简介：
The recent appearance of Zika virus (ZIKV) in Brazil should serve as a wake-up call to international authorities, as it poses a threat to global public health. In the present study, we investigated whether a mangrove plant, <i>Rhizophora mucronata</i> Lam. (<i>R. mucronata</i>) collected in Mauritius, possesses anti-ZIKV activity at the non-cytotoxic doses. ZIKVMC-MR766NIID (ZIKVGFP) was used for assessing anti ZIKV activity. In silico docking (Autodock 4) and molecular simulation were performed on collected data. Using a recombinant ZIKV expressing reporter green fluorescent protein(GFP) protein, we discovered that fruit and root methanolic, decocted fruit and root extracts were effective inhibitors of ZIKV infection in human epithelial A549 cells at negligible cytotoxicity. The mechanisms by which such extracts prevented ZIKV infection are linked to the inability of the virus to attach to the host cell surface. The outcomes of this study were supported by the docking calculations in which some of the dominant compounds have shown high binding affinity against ZIKV. The scientific data gathered in this study might pave the way for the future development of possible <i>R. mucronata</i> inhibitors to combat ZIKV.f Communicated by Ramaswamy H. Sarma
近期寨卡病毒（Zika virus, ZIKV）在巴西的出现，无疑为国际公共卫生主管机构敲响了警钟，因其对全球公共卫生构成了切实威胁。本研究针对从毛里求斯采集的红树植物红海榄（<i>Rhizophora mucronata</i> Lam.，简称<i>R. mucronata</i>）在非细胞毒性剂量下的抗ZIKV活性展开了探究。本研究采用ZIKVMC-MR766NIID（ZIKVGFP）毒株开展抗ZIKV活性评估。基于采集的实验数据，开展了虚拟对接（Autodock 4）与分子模拟研究。本研究使用表达报告基因绿色荧光蛋白（green fluorescent protein, GFP）的重组ZIKV毒株开展实验，结果发现，红海榄果实与根的甲醇提取物、煎煮提取的果实及根提取物，在细胞毒性可忽略不计的条件下，可有效抑制人上皮A549细胞中的ZIKV感染。此类提取物阻断ZIKV感染的作用机制，与病毒无法黏附宿主细胞表面相关。对接计算结果验证了本研究的结论：部分优势化合物对ZIKV展现出较高的结合亲和力。本研究获取的科学数据，可为未来开发针对ZIKV的红海榄源抑制剂奠定基础。本文由Ramaswamy H. Sarma转交。
