PY79 is a widely used laboratory strain of Bacillus subtilis. We sequenced the PY79 genome to high coverage with both the PacBio RSII and Illumina HiSeq2000 platforms. Initial assembly was performed u
tRNAs decoding standard 20 AA 86 Selenocysteine tRNAs (TCA) 0 Possible suppressor tRNAs (CTA,TTA,TCA) 0 tRNAs with undetermined or unknown isotypes 0 Predicted pseudogenes 0 Total tRNAs 86
Mutation accumulation was carried out in strains with and without DNA mismatch repair to determine the effect of sequence context on DNA polymerase error rate in vivo.
