Characterization of human erythroid chromatin in a two-phase liquid differentiation system

A two-phase liquid differentiation of peripheral blood mononuclear cells (PBMC) can be used to study human erythroid cells. To determine the epigenetic landscape of erythroid cells derived from PBMC we have performs ChIP-seq for histone marks associated with promoters (H3K4me3), enhancers (H3K4me1) and polycomb repression (H3K27me3) as well as for chromatin bound pol2. In addition we characterized the binding of the boundary element CTCF and key erythroid transcription factors NF-E2 and SCL/TAL. Overall design: Peripheral blood mononuclear cells were isolated and differentiated from a single male donor and a single replicate of ChIP-seq for histone marks (H3K4me1, H3K4me3, H3K27me3) and transcription factors (CTCF, NFE2, SCL/TAL, POL2). NG Capture-C was also performed on the same cells to determine the chromatin conformation of the alpha globin locus.