Cyclin E/A:Cdk2-mediated phosphorylation of p27/p21

The interaction between the Skp2 subunit of the SCF(Skp2) complex and p27 is dependent upon Cdk2:Cyclin A/E mediated phosphorylation of p27 at Thr 187 (Carrano et al, 1999; Tsvetkov et al, 1999). There is evidence that Cyclin A/B:Cdk1 can also bind and phosphorylate p27 on Thr 187 (Nakayama et al., 2004). This phosphorylation is also essential for the subsequent ubiquitination of p27.

SCF(Skp2)复合物中的Skp2亚基与p27的相互作用依赖于Cdk2:Cyclin A/E介导的p27在Thr 187位点的磷酸化（Carrano等，1999；Tsvetkov等，1999）。研究表明，Cyclin A/B:Cdk1亦能结合并磷酸化p27于Thr 187位点（Nakayama等，2004）。此磷酸化对于p27随后的泛素化亦至关重要。