Detection of duplication mutations in coding regions in normal lung and lung tumor in wild-type (WT) and FEN1 A159V mutant mice

Faithful and efficient Okazaki fragment maturation is the key to maintain the genome integrity. In addition, this process is coupled with deposition of histone proteins. Therefore, functional deficiency in Okazaki fragment maturation may cause duplication mutations. We conduct WES of normal lung and lung tumor from WT and A159V/WT mice to define the duplication mutations caused by defective Okazaki fragment maturation. We conduct whole exosome sequencing of the normal lung and tumor lung from WT and FEN1-A159V/WT mice (n=1 for each sample). We use Pindel to define the genome-wide duplication mutations frequency in the coding regions caused by defective Okazaki fragment maturation.