The gene expression of numerous SLC transporters is altered in the immortalized hypothalamic cell line N25/2 following amino acid starvation. Mus musculus

The immortalized mouse hypothalamic cell line N25/2 was treated with medium containing amino acids or medium lacking all amino acids for 1, 2, 3, 5 or 16 hours, and the gene expression was measured for 28270 genes using microarray. Numerous transporters from the SLC family were transcriptionally regulated in response to changed amino acid levels. Overall design: Singlet's were run in in each treatment group (controls vs. starved cells) for all times (1, 2, 3 and 16 hours), except for 5 hours with four replicates in each treatment group.