mRNA expression profile in WT and ZNF598 knockout HeLa cells stimulated with short poly I:C

mRNA expression profiles were compared between wild-type and ZNF598 knockout cells in the presence or absence of a RIG-I ligand, short poly I:C. WT and ZNF598 knockout HeLa cells were cultured in a 24-well-plate, and were then stimulated with or without 100 ng/ml of short poly I:C for 9 hours. Total RNA was extracted from stimulated and unstimulated HeLa cells, and was subjected to the microarray analysis.