Amplicon-Based Sequencing of Bat Coronaviruses from Ukraine
收藏NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP655602
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资源简介：
We analyzed fecal samples from bats in Ukraine using RT-PCR and amplicon-based Oxford Nanopore Technologies (ONT) sequencing. Nucleic acids were amplified via hemi-nested RT-PCR targeting the RdRp gene and sequenced with both ONT and Sanger methods. Alphacoronavirus 1 was detected, though low viral load and broad degenerate primers complicated the analysis of mixed strains.
创建时间：
2025-12-24
