Rearing density and dietary yeast on microbiota of tilapia. Effect of rearing density and dietary baker's yeast supplementation on microbiota of Nile tilapia

The effects of baker’s yeast as probiotics was evaluated in Nile tilapia reared at high density. Juvenile tilapia were distributed to tanks at high density (initial stocking density; 4.12 g/L) and fed with basal diet (CK) or diets supplemented with live (LY) or heat-inactivated yeast (HIY). Another group of fish reared at low density (initial stocking density; 2.06 g/L) and fed with basal diet was also included (LowCK). After 8 weeks of feeding,the gut wall and content of the hindgut were sampled for analysis of autochthonous and allochthonous microbiota.DNA was extracted. 16S rRNA gene V3-V4 amplicons of the microbial community were sequenced using pair-end method by Illumina Hiseq2500.