d18O profile for calcification depth estimate

δ18Oara profile for shelled pteropod calcification depth estimate based on seawater salinity and temperature measured by Argo floats. The oxygen isotopic composition of pteropod shells (δ18Optero) was compared to the δ18Oara at different depths. The calcification depth is defined as the depth where the values of δ18Optero and δ18Oara were equal within their respective uncertainties.Shelled pteropods are at risk from ocean acidification, with known effects on their shell durability and calcification. Pteropods typically form their aragonitic shells over specific depth ranges known as the ‘calcification depth’, which varies depending on species and habitats. Some Southern Ocean waters are already undersaturated with respect to aragonite and this could negatively affect shelled pteropods. However, the calcification depths of the pteropods have not been determined nor used to infer consequences of changing seawater carbonate chemistry in the Southern Ocean. In this study, we analysed the stable oxygen isotopic composition of Limacina rangii shells, collected by sediment traps in the subantarctic zone, to estimate their calcification depth. Shallow calcification depths (13-126 m) were detected in summer-autumn, while L. rangii calcified their shells deeper in the water column during winter-spring, with an average maximum calcification depth of 525 m. Recent shoaling of the aragonite saturation horizon is likely to negatively affect pteropods that calcify their shells in deep water. Such shoaling is likely to reduce shell formation and threaten population viability.