Identification and characterization of a core set of ROS wave-associated transcripts involved in the systemic acquired acclimation response of Arabidopsis to excess light [DPI]

Transcriptome profiling (RNA-seq) of local and systemic leaves of Arabidopsis Col treated with 50 mM DPI and exposed to a local treatment of high light To inhibit the propagation of the ROS wave from the local to the systemic leaf, a drop of 0.3% agarose-containing water or 50 mM DPI, was placed at the midpoint between the local and systemic leaves of 4– to 5-week-old Col-0 plants. Local tissue was then subjected to light stress for 8 min and both local and systemic leaves were used for RNA-Seq.