lnc-Cxcl2 suppressed Cxcl2 expression in mouse lung epithelial cells

In this study, we performed RNA-seq in GFP-VSV infected mouse lung epithelial cell lines (MLE-12 cells) and found that the expression of a non-coding RNA was increased upon VSV infection in Cxcl2 locus, which we termed it as lnc-Cxcl2. We further detected the function of lnc-Cxcl2 by performing transcriptome analysis in lnc-Cxcl2-deficient MLE-12 cells with and without VSV infection, and found that lnc-Cxcl2 suppressed Cxcl2 expression in MLE-12 cells with and without VSV infection. Overall design: RNA-seq was performed in non-infected and GFP-VSV infected-MLE-12 cells (each had two replicates), transcriptome analysis was performed in wild-type and lnc-Cxcl2-deficient MLE-12 cells with and without VSV infection for 12h.