Transcription inhibition in Aedes Albopictus C6/36 cells

To determine codon optimality in Aedes Albopictus C6/36 cells, we blocked transcription using three independent transcription inhibitors (5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB), Flavopiridol and Triptolide) and measured the RNA level at 6 hours post treatment using RNA-seq. RNA-seq was performed at 6 hours after mock treated (DMSO) or blocking transcription with Flavopiridol, Triptolide or DRB.