Behavioral data for: A preclinical model of THC edibles that produces high-dose cannabimimetic responses

No preclinical experimental approach enables the study of voluntary oral consumption of high-concentration Δ9-tetrahydrocannabinol (THC) and its intoxicating effects, mainly owing to the aversive response of rodents to THC that limits intake. Here we developed a palatable THC formulation and an optimized access paradigm in mice to drive voluntary consumption. THC was formulated in chocolate gelatin (THC-E-gel). Adult male and female mice were allowed ad libitum access for 1 and 2 h. Cannabimimetic responses (hypolocomotion, analgesia, and hypothermia) were measured following access. Levels of THC and its metabolites were measured in blood and brain tissue. Acute acoustic startle responses were measured to investigate THC-induced psychotomimetic behavior. When allowed access for 2 h to THC-E-gel on the second day of a three-day exposure paradigm, adult mice consumed up to ≈30 mg/kg over 2 h which resulted in robust cannabimimetic behavioral responses (hypolocomotion, analgesia and hypoth..., Animal Studies
Animal studies followed the guidelines established by AAALAC and were approved by IACUC of the University of Washington. Male and female C57BL/6J mice ranging from 8-14 weeks of age were used. Animals were housed with sibling littermates and were provided with standard chow and water, ad libitum, and without any additional environmental enrichment. Investigators were not blinded to experimental exposure conditions throughout assays due to the noticeable behavioral effects measured in response to THC. Animal procedures were approved by the Institutional Animal Care and Use Committee of the University of Washington and conform to the guidelines on the care and use of animals by the National Institutes of Health.
Pharmacological Agents
Animals received THC (0.1, 0.3, 1, 3, 5, 10, and 30 mg/kg) and SR141716 (SR1, 1 mg/kg) i.p. or were exposed to THC suspended in gelatin. THC and SR141716 (SR1) were provided by the National Institute of Drugs abuse Drug Supply Program (Bethesd..., , # Behavioral data for \"A preclinical model of THC edibles that produces high-dose cannabimimetic responses.

Data for the manuscript is organized here by their respective figures and panel labels.

## Description of the data and file structure

For ease of access of data when referencing the manuscript figures, all data is organized in excel files according to the figures they represent. Each figure shows individual data points plotted for interpretability and those respective data points are listed in the excel files for the figures. Main figures and supplementary data are separated into respective excel plots for the ease of access. All statistical analyses were performed in GraphPad Prism where data was plotted. Supplemental data is included in main figure data excel sheet. All data is included in the following outline:

Figure_1-dataset : **E-gel promotes heightened voluntary oral consumption of THC and induces cannabimimetic behaviors by adult mice.**

Tab 1: Panel c

Tab 2: Pane...