Gene expression profile at single cell level of ID8 ovarian cancer chemotherapy model

Chemotherapy is the first-line treatment for ovarian cancer, yet chemoresistance frequently develops at the late stage of treatment. In this study, we established the preclinical model of metastatic, syngeneic high-grade serous ovarian cancer (HGSOC) model using the ovarian cancer cell line ID8 that recapitulates the full chemotherapy course. 4-5 weeks following intraperitoneal injection, tumour grows extensively in the abdomen of the mice, which were subsequentially treated with cisplatin. Early treatment cycles significantly suppressed tumor growth, including a marked response during the first and subsequent cycles following initial relapse. Nevertheless, all tumors ultimately relapsed with chemoresistant phenotypes after two treatment cycles , mirroring the clinical course often observed in the treatment of HGSOC patients. We conducted single-cell RNA sequencing (scRNA-seq) on both parallelly debulked primary tumors and their corresponding resistant counterparts to elucidate changes in cellular composition. Primary tumor and chemoresistant tumor from the ID8 ovarian cancer chemotherapy model were harvested and analyzed using scRNAseq.