Rickettsia conorii subsp. heilongjiangensis strain:TIGMIC Genome sequencing and assembly. Rickettsia conorii subsp. heilongjiangensis strain:TIGMIC

A Rickettsia was isolated from Haemaphysalis longicornis collected from Anhui Province in 2019. Sequencing library of Rickettsia was then constructed using the AxyPrep Mag PCR clean up kit for MGI Tech Co., Ltd. Then it was prepared according to the Whole Genome Sequencing Library Preparation Protocol (DNBSEQ). The paired-end (PE) libraries were sequenced with a read length of 2 * 150 bp on a DNBseq-T7 platform at Grandomics Gene Technology Beijing Co. Ltd. (Beijing, China). Raw reads were filtered using the AfterQC v0.9.6 with default parameters. High-quality reads were aligned to the Chlorocebus sabaeus genome using the bowtie2 v2.4.1 with default parameters. The paired reads were discarded if one read matched to the Chlorocebus sabaeus genome by using samtools v1.9 with parameters -f 12. Genome assembly was accomplished by SPAdes v3.15.2. Binning and genome reconstruction were accomplished by MetaBAT2 v2:2.15.