Mapping the axolotl chromatin accessibility landscape by UUATAC-seq

The current understanding of regulatory sequences in the vertebrate genome is still incomplete. By introducing an adapter switching strategy, we developed an ultra-throughput ultra-sensitivity single-nucleus ATAC-seq (UUATAC-seq) protocol that enables construction of high-quality chromatin accessibility landscape from one species in a one-day experiment. By using UUATAC-seq, we mapped open chromatin regions for five representative vertebrate species at the landscape scale. UUATAC-seq was performed across 15 tissues from one axolotl to profile over 130,000 nuclei. Axolotl tissues were obtained from the d/d Ambystoma mexicanum strain (1-year old). Please note that the processed data file generated from all replicates is linked to the corresponding *_A sample records.