Figure S1 - Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos

Kinase dead Aurora and ZM447439 prevent cytokinesis. Confocal images of embryos previously injected with mRNA encoding kinase dead dominant negative Ci-Aurora (T193A/T247D/S248D), termed KD-Aurora. Microtubules are labeled with Map7::GFP (green) and chromosomes with histone H2B::Rfp1 (red). The effect of the KD-Aurora were indistinguishable from Aurora inhibition with 10 µM ZM447439. Both cause the appearance of multiple nuclei in the cells due to the inability of the cells to complete cleavage. n = 14, 3 animals for kinase dead Aurora (14/14 cytokinesis inhibited) and n = 45, 7 animals for ZM447439 (45/45 cytokinesis inhibited). Scale bars = 10 µm. (EPS)