Comparison of experimental approaches and conclusions in the present study and that of Davies et al., [20].

Key differences are our use of: a) both caveolin and flotillin as raft markers, b) a carefully controlled surface-labelling protocol, c) lysates from live cells treated with PI-PLC and d) the extensive use of chimera which ablate the purported GPI-anchoring motif. Asterisks denote the use of non-permeabilised cells without reference to controls. As we show elsewhere [21], fixative alone can cause significant cell permeabilisation.