Response of collecting duct cells to hypertonic stress

Transcriptional profile of inner medullary collecting duct cells in response to overnight NaCl hypertonic stress Overall design: To study the transcriptional response of inner medullary collecting duct cells to hypertonic stress, we treated 90% confluent inner medullary collecting duct cells with 100 mmol NaCl for 12 hrs and then extracted RNA for bulq RNA sequencing.