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Cytokine-mediated differentiation reflects genome-wide loss of a uSTAT transcriptional program

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NIAID Data Ecosystem2026-03-11 收录
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uSTAT transcriptional program HPC7 cells were fixed with either 1% formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of insterest after a pre-clearing step. Chromatin was washed, de-crosslinked, amplified, size selected by gel purification and sequenced.

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2019-05-15
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