Expression of FKBP5 in hippocampus of epileptic rats and its correlation with synaptic plasticity damage

AimTo observe the dynamic expression of FK-506 binding protein 5 (FKBP5) in the hippocampal tissue of epileptic rats and its correlation with synaptic plasticity damage.MethodsTwenty-four male SD rats were randomly divided into a control group and a model group, with 12 rats in each group. The epileptic model was induced by intraperitoneal injection of lithium chloride-pilocarpine, and the seizure latency was recorded. The level of seizures was assessed according the Racine grading standard, and cortical electroencephalograms were recorded. The levels of FKBP5 mRNA and protein in hippocampal tissues were detected by qRT-PCR and Western blotting. Golgi staining was used to observe the damage of neuronal dendritic spines in the hippocampus. Western blot was used to detect the protein levels of PSD95, BDNF, synapsin-1, and synaptotagmin-1.ResultsSD rats showed typical epileptic seizure behaviors after intraperitoneal injection of lithium chloridepilocarpine, accompanied by high-amplitude rhythmic spike and sharp wave patterns. Compared to the control group, the hippocampal FKBP5 protein levels in the model group significantly increased at 1, 4, and 24 h post-seizure (P P P P ConclusionsIntraperitoneal injection of lithium chloridepilocarpine can successfully induce epileptic models in rats, and the changed expression of FKBP5 in the hippocampus is involved in the mechanism underlying synaptic plasticity impairment in the hippocampus of epileptic rats.