Human feces metagenome 16s rDNA sequencing. human gut metagenome

The precise effects of HIV-1 on the human microbiome are unclear. Initial cross-sectional studies provided contradictory associations between microbial richness and HIV status and suggested shifts from Bacteroides to Prevotella predominance following HIV-1 infection, which have not been found in animal models or in studies matched for HIV-1 risk groups.This was a cross-sectional study where we first tested 129 HIV-1-infected subjects and 27 HIV-negative controls in Barcelona (BCN0). Findings were internally validated in 110 subjects from BCN0 providing a second fecal sample 1 month later. External validation was obtained in 77 HIV-1-infected and 7 non-infected subjects from Stockholm. In all study participants, we produced MiSeq 16S rRNA data on fecal microbiomes and collected comprehensive metadata.