The skeletal proteome of the feather star Anneissia japonica

Skeletal elements from the feather star Anneissia japonica were isolated and stripped of organic material. The skeleton was then demineralized and the occluded proteins isolated. The proteins were separated by SDS-PAGE and fractions were analyzed by LC-MS/MS. The results were compared to predicted proteins encoded by the genome. The proteins found in the organic matrix of the skeleton were compared to those found in other skeletons both within Echinodermata and to other taxa.