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Gene regulation by Pax6 in the lens placode - E9.5 (2). Mus musculus

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下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA213321
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资源简介:
Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Overall design: Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.
创建时间:
2013-07-25
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