Synergistic effects of space radiation and microgravity (miRNA WT and dys-mutant)

Space radiations and microgravity both could cause DNA damage in cells, but the effects of microgravity on DNA damage response to space radiations are still controversial. A mRNA microarray and microRNA microarray in dauer larvae of Caenorhabditis elegans (C. elegans) that endured spaceflight environment and space radiations environment during 16.5-day Shenzhou-8 space mission were performed. In our study, wild type and dys-1 mutant strains of C.elegans endured four conditions during shenzhou-8 spaceflight mission, including spaceflight static condition(ss), spaceflight 1-g centrifugal condition(sc), ground control condition(gc) and no-transport control. Limited to the quantity of worm samples, we performed technical-repeat test but not sample-repeat test. Accordingly, eight miRNA microarrays were performed.