Mus musculus Genome sequencing

The major type of protein arginine methyltransferase is PRMT1. Since the growth of embryos from Prmt1-/- mice was arrested shortly after implantation, PRMT1 must play a critical role in early mouse development. We used P300 and RXR ChIP-seq to investigate the effect of PRMT1 in early stem cell differentiation." PRMT1 knockdown D3 embryonic stem cells were generated (siPRMT) or as a control, scrambled sequence was introduced (siSCR). To avoid any feeder-cell contamination, the cells were passaged at least 3 times on 0.1% gelatin coated plates before the experiments. Samples were collected and used for P300 and RXR ChIP-seq from undifferentiated mouse embryonic stem cells and 24h atRA or DMSO treated cells.