Organoid fusion videos

Videos show the progression of fusion in 3CT organoids in round bottom 96 well plate wells over the course of approximately 24hrs. Mesenchymal cells were stained with Cell Tracker Orange, Epithelial cells were stained with Cell Tracker Green, and Endothelial Cells (not shown) were stained with Cell Tracker Blue. All treatments shown are controls; 0.1% DMSO in Co Culture Media (CnT-PRIME CC CELLnTEC) and were imaged on a confocal (Nikon A/C 2) by taking an image at a constant position (X, Y, and Z) at set intervals (15min for video 1 and 10min for Videos 2-6). After capture, a scale bar was applied, and images were compiled into a video at 10 frames per second in NIS elements (Nikon). In FIJI (ImageJ), the videos were stabilized using the image stabilizer plug-in and manually timestamped.