Transcriptome analysis of potential candidate genes and molecular pathways in colitis-associated colorectal cancer of Mkp-1-deficient mice

The nuclear phosphatase mitogen-activate protein kinase phosphatase-1 (MKP-1) is a key negative regulator of the innate immune response through the regulation of the biosynthesis of proinflammatory cytokines. In colorectal cancer (CRC), which is induced mainly by chronic inflammation, Mkp-1 overexpression was found in addition to disturbances in Mkp-1 functions, which may play a role in cancer development in different types of tumors. However, the potential molecular mechanisms by which Mkp-1 influences CRC development is not clear. Here, we performed global gene expression profiling of Mkp-1 KO mice using RNA sequencing (RNA-seq) to explore the role of Mkp-1 in CRC progression using transcriptome analysis. Two main categories including wild type that subdivided to three subgroups; wild type control (WT-C), wild type adenoma stage (WT-AS), wild type carcinoma stage (WT-CS), and Mkp-1 KO type that subdivided to three subgroups; Mkp-1 KO control (Mkp-1 KO C), Mkp-1 KO adenoma stage (Mkp-1 KO AS), Mkp-1 KO carcinoma stage (Mkp-1 KO CS).