RNA-sequencing of wild-type, CD97- and Galpa13-deficient splenic cDC2s

We analyzed the transcriptome differences of wild-type, CD97- and Galpha13-deficient (Adgre5-/- and CD11c-cre x Gna13fl/fl) type-2 conventional dendritic cells (cDC2s) in spleen. Three technical repeats of ~10^5 cells per sample from each of one mouse were included. Compared to wild-type cDC2s, CD97- and Galpha13-deficient cDC2s differentially expressed many genes, but CD97- and Galpha13-deficient cDC2s were almost the same. GSEA showed that Mrtf-a dependent genes were upregulated in CD97- and Galpha13-deficient cDC2s, which code for cytoskeleton proteins. These data support that CD97- Galpha13 signaling regulates splenic cDC2 motility by the actin cytoskeleton. Transcriptome analysis of WT, Adgre5-/- and CD11c-Cre Gna13fl/fl splenic cDC2s