The RNA polymerase II subunit B gene(POLR2B) functions as a growth regulator in human glioblastoma

Glioblastoma multiform (GBM) is the most common and aggressive brain tumor with dismissal prognosis. POLR2B encodes the second largest subunit of the RNA polymerase II, but its genomic status and function in GBM remains unclear. To explore POLR2B-dependent gene regulation, RNA-seq analysis was performed. Compared with the control group, POLR2B knockdown DBTRG-05MG cells showed differently expressed genes. mRNA profiles of DBTRG-05MG cells transfected with scramble shRNA (sh-Scb) or sh-POLR2B were generated by RNA sequencing, in double, using Illumina NovaSeq6000.