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Zea mays ssp. mexicana unigenes'sequences

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DataONE2016-03-01 更新2024-06-27 收录
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Due to lack of available reference genome, clean reads from 3 Zea mays ssp. mexicana were first de novo assembled into contigs using Trans-ABySS. After discarding the contigs <200 bp, the remaing contigs were then assembled into unigenes, whcih represented the individual transcriptome at the seedling stage. To obtain the transcriptomes from Zea mays ssp. mexicana, individual transcriptomes were pooled to reconstruct the transcriptome using CD-HIT-EST with a word length of 8 and an identity threshold ≥ 0.98. Sequentially, CAP3 was used to discard the redundancy transcripts, and the resulting contigs and singlets were combined to represent Zea mays ssp. mexicana transcriptome.
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2016-03-01
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