Use of Nanopores to Sequence Full-length Transcriptomes of Different Colored Potatoes and Study the Function of stAN1n

The presence of different colors in the plant kingdom has always been a focus of research in the field of plant science. In this study, nanopore sequencing technology was used to perform full-length transcriptome sequencing on the potato varieties Shepody, which has green leaves, and Redr3, which has red leaves. By analyzing and comparing the full-length transcriptome data of two different colored potato varieties, a total of 2715 significantly differently expressed genes were found. At the same time, a transcript of the AN1 gene was found and named stAN1n. The overexpression of stAN1n in tobacco caused tobacco plants to change from green to purple, and tobacco stalks became thinner and softer. Further analysis showed that stAN1n is likely to interact with FIZZY-RELATED 3 and Histone H4 to form a protein complex, which participates in activation of the expression of the downstream anthocyanin genes ANS and DFR. In addition, it was also found that the disease-resistance genes associated with stAN1n and FIZZY-RELATED 3 in Redr3 were down-regulated, indicating that the overexpression of stAN1n would reduce the disease resistance of plants.