Data for Figures 3 A, C, D

G- growth affects cell fate choice due to changes in cell cycle dynamics A. G- cells have a longer G2 phase. S and G2 phase lengths were determined from PCNA localization (Muramoto and Chubb, 2008). Only total cell cycle length and G2 phase length of G- grown cells are significantly longer. C. G- cells are biased towards prestalk cell fate. The percentage of prestalk and prespore cells was significantly different in G+ and G- cells in the low-density differentiation assay (t-test; p=5.5 x 10-8, p=6 x 10-7, p=3 x 10-7). D. Stalk cell propensity increases in G- cells with a longer than average cell cycle length Percentages of prespore and prestalk cells were plotted for G+ cells (solid line, see Figure 2D) and G- cells (dashed line) at different cell cycle positions. G- cells with a longer than average cell cycle (>8 hours) showed a much higher propensity to differentiate into prestalk cells.