Comparative transcriptome analysis between two tomato materials under drought stress

RNA sequencing was performed to investigate the the response mechanism of tomato response to drought stress. C2H2-type zinc finger proteins are classic and extensively studied members of the zinc finger family. C2H2-type zinc finger proteins participate in plant growth, development and stress responses. In this study, 99 C2H2-type zinc finger protein genes were identified and classified into four groups, and many functionally related cis-elements were identified. Differential C2H2-ZFP gene expression and specific responses were analyzed under drought, cold, salt and pathogen stresses based on RNA-Seq data. Thirty-two C2H2 genes were identified in response to multiple stresses. Seven, 3, 5, and 8 genes were specifically expressed under drought, cold, salt and pathogenic stresses, respectively. Five glycometabolism and sphingolipid-related, pathways and the endocytosis pathway were enriched by KEGG analysis. The results of this study represent a foundation for further study of the function of C2H2-type zinc finger proteins and will provide us with genetic resources for stress tolerance breeding. Overall design: mRNA profiles of different tomato leaf samples were generated by deep sequencing. The sequence reads that passed quality filters were analyzed at the transcript . Please note that the 'Micro-TOM-EX' is the 'Micro-TOM' plants overexpressing a GATA transcription factor gene. CK0*_0h = Micro-TOM_control CK3*_3h = Micro-TOM_drought stress for 3hrs CK6*_6h = Micro-TOM_drought stress for 6hrs EX0*_0h = Micro-TOM-EX_control EX3*_3h = Micro-TOM-EX_drought stress for 3hrs EX6*_6h = Micro-TOM-EX_drought stress for 6hrs