In vitro antibody analysis of sera from rhesus monkeys immunized with BDES-PfCSP.

aThe anti-PfCSP Ab and anti-NANP titers of sera obtained from immunized monkeys were evaluated by ELISA. Means ± SD are shown (n = 6).bViable PfCSP-Tc/Pb sporozoites (5×103) were incubated with pooled serum from BDES-PfCSP-immunized monkeys for 40 min on ice, and then added to cultured HepG2 cells. P. berghei 18S rRNA was measured by qRT-PCR 72 h after incubation. Data represent the means ± SD of the % sporozoite invasion efficacy values from cultures performed in triplicate for each group. Controls included sporozoites incubated with 25 mg/ml 2A10 mAb. Compared with the non-immune serum, all samples significantly reduced the number of the 18 S rRNA copies (*, pi.m.: intramuscular, s.c.: subcutaneous, i.d.: intradermal.