Transcriptional profiling of cochlear neurons

In order to characterize the various subpopulations of neurons present in the inner ear, we dissected out the cochleas from wild-type mice, removing non-neuronal populations as possible, then dissociated the tissue to obtain a single cell suspension and profiled these by single cell RNA-Seq. This study's goal was to characterize the native populations of murine cochlear neurons. To ensure reproducibility of our findings, neurons from 4 separate mice were profiled.为了表征内耳中存在的各类神经元亚群，我们从野生型小鼠体内解剖分离耳蜗，尽可能剔除非神经元细胞群，随后将组织解离以获取单细胞悬液，并通过单细胞RNA测序（single cell RNA-Seq）对其进行转录组分析。本研究旨在表征小鼠耳蜗神经元的天然细胞群。为确保研究结果的可重复性，我们对来自4只独立小鼠的神经元开展了测序表征。