Affymetrix Mouse liver gene expression profiling of Cross B6/J x A/J. Mus musculus

Summary Mapping the genetic architecture of RNA transcript abundances has potential for revealing molecular mechanisms underlying complex traits like disease. Gene expression microarrays allow monitoring genetic control on a whole transcriptome level, but validity and reproducibility of microarray data is a subject of ongoing scientific debate. Here we demonstrate how combining genetic and gene expression data provide a basis for biologically relevant assessment of the current state of microarray technology. We profile liver samples from a murine F2 intercross population using Affymetrix microarrays. Overall design: Tissue profiling in a mouse F2 cross. We analyzed 294 Liver samples.