In search for materials able to be colonized by a normal endothelium: сharacterization and NGS gene expression profiling of human primary endotheliocytes cultivated on electrospun 3D matrices

Endothelization of the luminal surface of vascular grafts is required for their long-term functioning. In order to select the best surface we have cultivated HUVEC on different 3D matrices and assessed the efficacy of cell proliferation and the changes in the gene expression profiles. 3D matrices were produced by electrospinning from solutions of poly(D,L-lactide-co-glycolide) (PLGA), polycaprolactone (PCL) and blends of PCL with gelatin (PCL-Gl) and glutaraldehyde-treated PCL-GL (PCL-Gl-glu) in hexafluoroisopropanol. Gene expression profiling was executed using Illumina HiSeq2500 platform. RNA-Seq gene expression profiling of HUVEC cells grown on different 3D matrices. We used HUVEC cells obtained from two donors, thus we obtained two biological replicates for each matrix tested (namely, control/tissue culture polystyrene (TCPS), PCL, PLGA, PCL-Gl, PCL-Gl-glu) and generated 10 RNA-Seq gene expression profiles in total.